Skip to main content
. 2015 Oct 23;3:169. doi: 10.3389/fbioe.2015.00169

Figure 3.

Figure 3

Relative mRNA expression levels were assessed by qPCR. The basal mRNA expression levels were determined for hfMSCs from monolayer culture on tissue culture-treated polystyrene (Day 0). (A,B) ALCAM and CD63, both genes associated with the maintenance of stemness, did not show significant differences between the basal gene expression levels and the expression levels after 9 days of static or biaxial bioreactor culture. (C) F-Actin showed a slight down-regulation after 9 days in static culture and a significant up-regulation after 3 days static followed by 6 days biaxial bioreactor culture. This same trend was found for Runx2 (I). (G) ALP was down-regulated for both static and biaxial bioreactor culture. (H,D) ACAN and Sox9 were down-regulated in convection culture. (E,F) Col-1 and col-2, markers for ECM production, were down-regulated in time in static culture, but retained basal expression levels when the scaffolds were placed in biaxial bioreactor culture [n = 4 except for day 9 dynamic (n = 5), *p < 0.05, **p < 0.01, ***p < 0.001].