TABLE 1.
Effect of global ACAT2 and intestine-specific MTP gene deletion on tissue and plasma lipids in chow- and Western diet-fed mice
| Chow Fed | Western Fed | ||||||||
| WT | Soat2−/− | I-Mttp−/− | I-DKO | WT | Soat2−/− | I-Mttp−/− | I-DKO | Interaction (P) (Genotype vs. Diet) | |
| Intestine (μg/mg protein) | |||||||||
| Triglycerides | 16.8 ± 3.0a,r | 20.7 ± 3.4a,r (+23.2) | 730.2 ± 257.6b,r (+4246.4)*** | 831.1 ± 221.0b,r (+4847.0)*** | 44.4 ± 4.2a,r | 46.3 ± 6.2a,r (+4.3) | 319.2 ± 40.0b,s (+618.9)*** | 375.9 ± 40.9b,s (+746.6)*** | <0.001 |
| Total cholesterol | 16.6 ± 2.5a,r | 18.3 ± 2.5a,r (+10.2) | 19.3 ± 2.2a,r (+16.3)* | 18.3 ± 4.8a,r (+10.2) | 23.9 ± 1.8a,s | 24.2 ± 1.8a,s (+1.3) | 28.7 ± 0.5b,s (+20.1)* | 21.2 ± 0.9a,r (−11.3) | NS |
| Free cholesterol | 9.8 ± 1.2a,r | 14.3 ± 1.7b,r (+45.9)*** | 12.6 ± 1.8b,r (+28.6)** | 14.9 ± 3.8b,r (+52.0)** | 10.8 ± 1.9a,r | 17.8 ± 0.7b,r (+64.8)** | 13.6 ± 1.4c,r (+25.9) | 18.5 ± 1.0b,r (+71.3)** | NS |
| Esterified cholesterol | 6.8 ± 2.1a,r | 3.9 ± 1.5b,r (−42.6)** | 6.7 ± 1.3a,r (−1.5) | 3.4 ± 1.5b,r (−50.0)** | 13.1 ± 0.7a,s | 6.5 ± 1.2b,r (−50.4)** | 15.1 ± 1.9a,s (+15.3) | 2.7 ± 1.2c,r (−79.4)** | <0.001 |
| Liver (μg/mg protein) | |||||||||
| Triglycerides | 25.6 ± 6.6a,r | 26.4 ± 3.5a,r (+3.1) | 13.7 ± 2.5b,r (−46.5)*** | 16.3 ± 3.9b,r (−36.3)** | 460.9 ± 60.5a,s | 183.9 ± 34.0b,s (−60.1)** | 444.5 ± 44.5a,s (−3.4) | 135.3 ± 26.0b,s (−70.6)** | <0.001 |
| Total cholesterol | 8.0 ± 0.8a,r | 5.6 ± 0.9b,r (−30.0)*** | 9.7 ± 1.0c,r (+21.3)** | 9.3 ± 1.9c,r (+16.3) | 17.1 ± 0.6a,s | 7.3 ± 0.3b,r (−57.3)*** | 18.1 ± 0.9a,s (+5.8) | 8.0 ± 1.1b,r (−53.2)*** | <0.001 |
| Free cholesterol | 5.2 ± 0.6a,r | 5.1 ± 0.8a,r (−1.9) | 4.7 ± 0.7a,r (−9.6) | 6.1 ± 0.9b,r (+17.3)* | 4.1 ± 0.3a,r | 3.5 ± 0.4a,s (−14.6)* | 6.4 ± 0.7b,s (+56.1)** | 3.5 ± 0.3a,s (−14.6)* | <0.001 |
| Esterified cholesterol | 2.8 ± 0.9a,r | 0.5 ± 0.5b,r (−82.1)*** | 4.9 ± 1.0c,r (+60.7)*** | 3.2 ± 1.2a,r (+14.3) | 13.0 ± 0.6a,s | 3.9 ± 0.1b,s (−70.0)*** | 11.7 ± 0.5a,s (−10.0)* | 4.5 ± 1.2b,r (−65.4)*** | <0.001 |
| Plasma (mg/dl) | |||||||||
| Triglycerides | 41.7 ± 6.1a,r | 46.5 ± 5.2a,r (+11.5) | 22.5 ± 5.6b,r (−46.0)c | 27.8 ± 6.3b,r (−33.3)c | 60.4 ± 6.1a,s | 82.2 ± 6.6b,s (+36.1)a | 42.3 ± 5.1c,s (−30.0)a | 102.2 ± 6.0d,s (+69.2)b | <0.001 |
| Total cholesterol | 83.7 ± 6.4a,r | 68.4 ± 2.5b,r (−18.3)*** | 37.4 ± 10.9c,r (−55.3)*** | 38.1 ± 5.5c,r (−54.5)*** | 132.3 ± 10.6a,s | 84.7 ± 5.5b,s (−36.0)** | 63.3 ± 6.5c,s (−52.2)*** | 46.7 ± 3.8d,r (−64.7)*** | <0.001 |
| Free cholesterol | 20.0 ± 1.4a,r | 19.6 ± 1.9a,r (−2.0) | 10.5 ± 1.2b,r (−47.5)*** | 10.0 ± 3.3b,r (−50.0)*** | 44.7 ± 4.4a,s | 37.7 ± 2.3b,s (−15.7) | 34.7 ± 2.5b,s (−22.4)* | 32.3 ± 1.8b,s (−27.7)* | <0.05 |
| Esterified cholesterol | 63.7 ± 6.1a,r | 48.8 ± 3.9b,r (−23.4)*** | 26.9 ± 10.5c,r (−57.8)*** | 28.4 ± 6.0c,r (−55.4)*** | 87.6 ± 8.3a,s | 47.0 ± 5.4b,r (−46.3)** | 28.5 ± 6.0c,r (−67.5)*** | 14.3 ± 2.5d,s (−83.7)*** | <0.001 |
Intestinal and hepatic tissues and plasma from WT, Soat2−/−, I-Mttp−/−, and I-DKO mice fed either chow (n = 8) or Western (n = 3) diet were used to measure triglycerides, total, free, and esterified cholesterol mass. Data are presented as mean ± SD. Values in parenthesis show percent change compared with WT. Significant differences (a, b, c, d) within chow and Western diet groups were determined by one-way ANOVA using Neuman-Keuls multiple comparison test. Differences (r, s) between chow and Western diet groups were determined by two-way ANOVA with Bonferroni posttest.
*P < 0.05 compared with WT mice as determined by Student’s t-test.
**P < 0.01 compared with WT mice as determined by Student’s t-test.
***P < 0.001 compared with WT mice as determined by Student’s t-test.