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Kinetic analysis of 20-HETE analysis by UGT1A3, UGT1A9, and UGT2B7 isoforms
| Kinetic Parameters | |||
| UGT Isoform | Km (μM) | Vmax (nmol/min/mg protein) | CLint (Vmax/Km) (μl/min/mg protein) |
| UGT1A3 | 78.4 | 1.33 | 17.0 |
| UGT1A9 | 22.2 | 1.78 | 84.5 |
| UGT2B7 | 14.8 | 1.62 | 109.6 |
The best fit of data was determined by the examination of required parameters using SigmaPlot 8.0. The formation of 20-HETE glucuronide showed a fit to Michaelis-Menten kinetics. Details are described in the Experimental Procedures.
