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. 2015 Oct 21;9:422. doi: 10.3389/fncel.2015.00422

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Copyright © 2015 Caputo, Piano, Demontis, Bacchi, Casarosa, Della Santina and Gargini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY. The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Co-immunoprecipitation of TMEM16A (input) with the α₁ subunit of calcium channels revealed by either an anti-Panα1 (output, A) or anti-Cav1.4 (output, B) antibodies. The tsA-201 cell line was co-transfected with TMEM16A, Cav1.4, β₂, and constructs encoding either WT (Cacna2d4^wt, left panels) or mutant (Cacna2d4^mut, right panels) α₂δ₄ variants. WL, whole lysate; SUR, supernatant; IP, immunoprecipitate. Representative example of N = 3 experiments.