Cell survival studies and LC50 determination. Survival of Hela and HeLa HSV1-mNLS-sr39TK cells grown in the presence of increasing amounts of either 5 (3A, top) or 6 (3B, bottom). Cells grown in the presence of vehicle alone served as negative control; data for cell survival in the presence of either 5 or 6 was plotted as a percent of vehicle control. LC50 (μM): 3A.
5, HeLa: >> 100 μM, HeLa HSV-1-mNLS-sr39TK: 0.00078 μM; 3B. 6, HeLa: >> 100 μM, HeLa HSV-1-mNLS-sr39TK: 0.074 μM). Each point represents the mean value of sextuplicate determinations; bars represent ± standard deviation when larger than symbol.