(A) Schematic diagram of Col1A1 MT-DLX3 TG construct (5 kb) containing mouse 2.3 kb type I collagen promoter, 2.7 kb mouse MT-DLX3 cDNA, and Bgh poly A tail (pA). Col1A1SS1 and AS4 primers were used for the PCR genotyping. (B) XbaI digested chromosomal DNA from tail biopsies was used for genomic southern blot analyses. Three TG lines (TG1, TG2, and TG3) were identified (Con: non transgenic control). The 5 kb transgene bands were detected in TG1, TG2 and TG3.