Figure 1. Media pH changes over incubation times from mDCR and mDCR+MES media incubated under dark and light conditions.

Medium pH was pre-adjusted to 3.6, 5.1, 5.7, 6.3, and 7.8 prior to adding 7 g/L of agar, MES and autoclave. Medium was incubated in a growth chamber with full light or darkness at 25ºC until it reached incubation requirement. Post-autoclaving pH was recorded using a pH meter at each incubation time. Datapoints represent mean pH (n=5 for each datapoint), and were fitted with linear regression lines. Please see Dataset 1 for the raw data.