TABLE 2.

Chemical composition of murine HDL

	WT	LDLR−/−−/−	P
	% of Total Mass	% of Total Mass
Cholesterol	21.1 ± 0.3	20.4 ± 0.4	0.25
Phospholipids	28.0 ± 0.6	24.2 ± 0.5	0.0039
Triglycerides	3.4 ± 0.07	7.9 ± 0.1	<0.0001
Protein	47.5 ± 0.9	47.6 ± 1.0	0.98

^−/−Blood was harvested in parallel from fasted (4 h) WT or LDLR^−/− male mice. Subsequently, from plasma, HDL (d = 1.063–1.21 g/ml) was isolated by sequential ultracentrifugation. Thereafter, cholesterol, phospholipids, triglycerides, and protein were analyzed. Values are means ± SEM of n = 5 independent determinations; four independent preparations yielded qualitatively identical results.