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TLC analysis of the total lipids extracted from the haploid mutant P. pastoris strain expressing CpFAH and CpDGAT1. 1, Castor oil; 2, D12-KO; 3, CpFAH/D12-KO; 4, CpFAH+CpDGAT1/D12-KO; 5, ricinoleic acid standard (FRA); 6, TLC standard. DAG, diacylglycerol; FRA, free ricinoleic acid.
