TABLE 1.

Fatty acid compositions of Pichia GS115 (wild type), D12-KO (defective in the Δ12 desaturase), CpFAH/D12-KO (CpFAH alone), and CpFAH+CpDGAT1/D12-KO (coexpression) at day 3 of induction

Fatty Acid	GS115	D12-KO	CpFAH/D12-KO	CpFAH+CpDGAT1/D12-KO
16:0	14.38 ± 0.35	10.52 ± 0.10	7.7 ± 0.11	7.5 ± 0.50
16:1	4.33 ± 0.19	4.75 ± 0.36	7.6 ± 0.49	6.9 ± 0.11
17:0	0 ± 0.00	0.56 ± 0.06	0.5 ± 0.06	0.4 ± 0.06
17:1	0 ± 0.00	3.52 ± 0.04	1.3 ± 0.13	1.5 ± 0.56
18:0	1.32 ± 0.11	1.57 ± 0.26	0.6 ± 0.11	0.8 ± 0.15
18:1–9	50.71 ± 1.56	77.85 ± 0.04	17.2 ± 1.55	14.1 ± 2.06
18:1–11	0 ± 0.00	1.23 ± 0.06	1.1 ± 0.12	0.9 ± 0.25
18:2	22.81 ± 1.07	0 ± 0.00	10.5 ± 0.06	10.2 ± 2.14
18:3	6.46 ± 0.30	0 ± 0.00	2.1 ± 0.31	2.0 ± 0.05
18:1-OH	0 ± 0.00	0 ± 0.00	51.3 ± 2.18	55.7 ± 0.94

18:1-OH, ricinoleic acid. Results were calculated from the peak area of FAMEs. Values are means of three replicates with SD.