Fig. 3.

Dilysyl-MDA cross-links are formed in activated human washed platelets in a thromboxane synthase-dependent way. Human washed platelets were preincubated with vehicle (subject) or either with 100 µM ozagrel (Oz), 100 µM aspirin (Asp), or 1.5 mM SA or 3-MoSA for 40 min. After incubation, 5 µM arachidonic acid (AA) was added for 2 h. A: Dilysyl-MDA cross-links were purified and quantified by isotopic dilution using LC/MS/MS. The product ions at m/z 294 for dilysyl-MDA cross-link and m/z 306 for the IS were monitored. * P < 0.0001 versus all other conditions. B: As a measure of COX activity, TxB₂ was determined by GC/MS from 50 µl of washed platelet. ** P < 0.0001 versus subject, Oz or Asp. One-way ANOVA followed by Tukey’s multiple comparisons test was used (n > 6).