Fig. 5.

Scavenger-MDA adducts are formed when human washed platelets are activated in presence of scavengers. Human washed platelets were preincubated with MoSA or SA (1.5 mM final concentration) for 40 min, followed by arachidonic acid (AA; at 5 µM final concentration) for an additional 2 h. Scavenger-MDA adducts were extracted with ethyl acetate and analyzed by LC/MS/MS by monitoring the specific SRM transitions of m/z 208→136 (SA-MDA) and 178→106 (MoSA-MDA). ⁺ P < 0.0001 versus subject or AA. One-way ANOVA followed by Tukey’s multiple comparisons test was used (n > 7).