Fig. 4.

The effect of pro- and antiobesogenic treatments on LD formation and accumulation during adipogenesis. A: Smoothed distribution of LD diameter sizes in terminally differentiated SGBS cells in the presence and absence of 10 µmol/l DHA treatment over a 14 day period (n = 7,444 LDs in eight images). B: The Oil Red O dye extractions were measured spectrophotometrically at 520 nm (OD_520nm) in terminally differentiated SGBS cells in the presence and absence of 10 µmol/l DHA treatment over a 14 day period. Data are shown as mean ± SD (three biological replicates with two technical replicates per sample). C: Representative microscopy images of control and 10 µmol/l DHA-treated SGBS cells on day 14. For these and panels F, I, and L, images are only representative of a small portion within a single image. D: The change of smoothed distribution of LD diameter sizes in differentiating SGBS cells in low and high glucose concentrations for a total of 14 days (n = 5,955 LDs). E: The Oil Red O dye extractions were measured spectrophotometrically at 520 nm (OD_520nm) in differentiating SGBS cells in low and high glucose concentrations for a total of 14 days. Data are shown as mean ± SD (four biological replicates with two technical replicates per sample). F: Representative microscopy images of SGBS cells fed in low- and high-glucose-concentrated media up to day 14. G: The change of smoothed distribution of LD diameter sizes in differentiating SGBS cells on days 8 and 12 after a 48 h treatment with or without the 10 µg coated ZnO nanoparticles on day 6 (n = 33,626 LDs). H: The Oil Red O dye extractions were measured spectrophotometrically at 520 nm (OD_520nm) in differentiating SGBS cells on days 8 and 12 after a 48 h treatment with or without the 10 µg coated ZnO nanoparticles on day 6. Data are shown as mean ± SD (two biological replicates with two technical replicates per sample). I: Representative microscopy images of SGBS cells at day 14 after a 48 h treatment with or without a 48 h treatment the 10 µg coated ZnO nanoparticles on day 6. J: Smoothed distribution of LD diameter sizes in SGBS cells differentiated in the presence and absence of 2 µmol/l roziglitazone until day 14 (n = 33,749 LDs). Vertical lines: 95th percentile; full lines: day 10; dashed lines: day 14. K: The Oil Red O dye extractions were measured spectrophotometrically at 520 nm (OD_520nm) in SGBS cells differentiated in the presence or absence of 2 µmol/l roziglitazone until day 14. Data are shown as mean ± SD (three biological replicates with two technical replicates per sample). L: Representative microscopy images of SGBS cells in the presence and absence of 2 µmol/l roziglitazone for a total of 14 days.