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. 2015 Oct 10;23(11):880–892. doi: 10.1089/ars.2014.6070

FIG. 5.

FIG. 5.

ACE2 activation restores endothelial function in db/db mice. The effect of chronic treatment with diminazene aceturate (DIZE, 15 mg/kg/day, 14 days) on the (A) activity and (B) expression of ACE2 in db/db mouse aortas. RFU, relative fluorescence unit. (C) Plasma Ang (1-7) level in db/db mice with and without DIZE treatment. Data are mean±SEM of five to six mice. *p<0.05 versus vehicle. (D) ACh-induced EDRs and (E) SNP-induced relaxations in aortas and (F) FMD in mesenteric arteries from db/db mice. Data are mean±SEM of six mice. *p<0.05 versus vehicle. (G) EDRs in db/db mouse aortas following a 24-h exposure to DIZE (100 μM), or DIZE plus ACE2 inhibitor, DX600 (1 μM), or DIZE plus Mas receptor antagonist, A779 (1 μM). Data are mean±SEM of five to six mice. *p<0.05 versus DIZE. EDRs in aortas from (H) ACE2 WT or (I) ACE2 KO mice following a 48-h exposure to normal glucose (NG, 5 mM) high glucose (HG, 30 mM), HG plus DIZE (100 μM), and HG plus Ang (1-7) (1 μM). Data are mean±SEM of five mice. (H) *p<0.05 versus HG, (I) *p<0.05 versus NG, #p<0.05 versus HG.