Fig. 3.

Validation of anther/pollen specific expression of lncRNAs by qRT-PCR. a Gel image of RT-PCR products of ten randomly selected anther/pollen specific lncRNAs. b to g The expression of six lncRNAs quantified by qRT-PCR (black bar and Y-axis on the left). Error bar indicates standard deviation (SD) of two biological replicates with three technical replicates each. The relative FPKM of the same six lncRNAs based on RNA-seq data was also shown (red line and Y-axis on the right). Gene11892 was used as the internal control for both RNA-seq (red line) and qRT-PCR (black bar). RNAs were from anthers at stage7/8, stage9, stage10, stage11, and stage12 as well as mature pollen