Fig. 1.

XNJ inhibits p53 transcriptional activity. a Serum-free condition induced the p53 transcriptional activity. PC12 cells were transfected with p53 promoter reporter construct and then stimulated with serum or serum-free condition for 6, 12, 24 and 36 h respectively. b p53 inhibitor, pifithrin α, inhibited p53 transcriptional activity. PC12 cells were transfected with p53 promoter reporter construct and then stimulated with serum or serum-free condition for 12 h in the absence or presence of pifithrin α. c XNJ inhibited p53 transcriptional activity. PC12 cells were transfected with p53 promoter reporter construct and then stimulated with serum or serum-free condition for 12 h in the absence or presence of XNJ. Luciferase activity was determined in cell lysates and normalized to Renilla activity. Results are representative of three independent experiments in duplicate