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. Author manuscript; available in PMC: 2016 Sep 17.
Published in final edited form as: Mol Cell. 2015 Sep 17;59(6):1025–1034. doi: 10.1016/j.molcel.2015.08.012

Figure 1. Purification of TFIIH and Core7.

Figure 1

(A) An illustration of TFIIH complex (Zhovmer et al., 2010) with Core7 colored yellow and CAK blue.

(B) Purified TFIIH and Core7 (5 μg each) were analyzed on a 4%–12% Bis-Tris NuPAGE SDS gel and stained with Coomassie blue.

(C) Samples of Core7 purification steps (5 μg each), crude extract (1), and eluent from Heparin (2), M2 beads (3), HisTrap (4), Superose 6 (5), are shown with the molecular weight markers (M) on a 4%–12% SDS-PAGE gel.