Figure 2. ATPase Activity of TFIIH and Core7.
(A) ATPase activities of TFIIH and Core7 were assayed alone or in the presence of 50-fold molar excess of ssDNA (20 nt) or dsDNA (25 bp) (Table S1) and shown in the bar graph.
(B) ATPase activities of Core7 in the presence of ssDNA, dsDNA, or bubble-structured DNA of variant lengths.
(C) ATPase activities of WT, XPBKR, XPDKR, and double KR (2KR) mutant Core7 as protein alone or with different ssDNAs and different bulky lesions.
(D) ssDNA, with or without an AP lesion, stimulated the Core7 ATPase equally. Standard deviations were estimated based on at least triplicates.
