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. Author manuscript; available in PMC: 2016 Sep 17.
Published in final edited form as: Mol Cell. 2015 Sep 17;59(6):1025–1034. doi: 10.1016/j.molcel.2015.08.012

Figure 6. Effects of CAK on DNA Binding, Unwinding, and Dual Incision.

Figure 6

(A) TFIIH, WT, and two mutant Core7 were assayed for their ability to activate dual incisions in the reconstituted NER reaction with a 6-4 photoproduct (6-4 PP). The amount of each protein was equalized based on anti-XPB immunoblotting (IB).

(B) EMSA of ssDNA binding by TFIIH and WT Core7 (0, 0.25, 0.5, 1, 2, 4, 8, and 16 nM). The black arrow indicates protein-DNA complexes of Core7 and TFIIH.

(C) DNA helicase activities of Core7 and TFIIH in the presence and absence of XPA.

(D) EMSA examination of Core7 and TFIIH recruitment by XPC/RAD23B/Centrin-2 to a preformed DNA bubble (bubble7, Table S1). DNA complexes with XPC, TFIIH, or Core7 are marked. Supershift indicates the XPC-TFIIH-DNA and XPC-Core7-DNA complexes.