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. Author manuscript; available in PMC: 2015 Oct 23.
Published in final edited form as: J Mol Biol. 2008 Apr 9;380(1):51–66. doi: 10.1016/j.jmb.2008.03.076

Figure 7.

Figure 7

Creation of immuno-MVs via ClyA-scFv chimeras. (a) Fluorescence microscopy of whole cells and vesicles generated from JC8031 cells expressing scFv.Dig, ClyA-scFv.Dig or Lpp-OmpA-scFv.Dig as indicated. For these studies, cells were grown and induced at room temperature followed by fluorescent labeling of cells or their derived vesicles with 1 µM Dig-BODIPY for 1 h at room temperature. (b) Genetic analysis of scFv.Dig localization was performed using flow cytometric analysis of strains and plasmids as indicated. Cells were grown and induced at room temperature followed by labeling with 1 µM Dig-BODIPY for 1 h at room temperature. Fluorescence is reported as the mean fluorescence for each cell population and was assayed in triplicate with error bars showing ± standard error.