. Author manuscript; available in PMC: 2015 Oct 23.

Published in final edited form as: J Mol Biol. 2008 Apr 9;380(1):51–66. doi: 10.1016/j.jmb.2008.03.076

Table 1.

ClyA-mediated display of enzymatically active proteins

Construct	Cell surface activity	OMV activity
β-lactamase (Bla)	[ΔA486/min]	[ΔA486/min]
pBAD18-Kan	0.03	0.02
pΔss-Bla	0.47	0.98
pClyA-Bla	8.53	7.05
pBla-ClyA	1.43	1.65
Organophosphorus hydrolase (OPH)	[U*1000/OD₆₀₀]	[U*1000/g total protein]
pBAD24	0.09	0.04
pOPH	0.36	1.63
pClyA-OPH	11.62	81.63
pOPH-ClyA	0.22	8.19
β-galactosidase (LacZ)	[ΔA420/min]	[ΔA420/min]
pBAD24	0.02	0.02
pLacZ	0.02 (7.21)^†	0.03
pClyA-LacZ	0.04 (8.33)^†	0.03

^†

Values in parentheses represent activity in the cytoplasmic fraction.

All values represent the average of 3 replicate experiments where the standard error was <5%.