Table 3.
MS/MS Characterization of The Six Protein Markers in Proteomic Signature Model of HCC.
| Spot | Protein identity (Gene name) | MW (kDa) | pI | Mascot Score |
Matched Peaks |
Sequenced Peptides | Accession Numbers |
|---|---|---|---|---|---|---|---|
| SSP8103 | Haptoglobin (Hp2) | 41,743 | 6.23 | 173 | 1311.6 1439.6 895.5 |
TEGDGVYTLNDK TEGDGVYTLNNEK NPANPVQR |
1006264A |
| SSP0026 | Cytochrome b5 (CYB5A) | 15,330 | 4.88 | 76 | 1186.6 1134.59 1511.73 |
YYTLEEIQK STWLILHHK FLEEHPGGEEVLR |
P00167 |
| SSP0023 | Progesterone receptor membrane component 1 (PGRMC1) | 21,671 | 4.56 | 85 | 1644.8 1516.7 |
KFYGPEGPYGVFAGR FYGPEGPYGVFAGR |
NP_006658 |
| SSP6108 | Heat shock 27kDa protein 1 (HSPB1) | 22,782 | 5.98 | 73 | 961.4 1163.62 1104.53 |
GPSWDPFR LFDQAFGLPR QDEHGYISR |
NP_001531 |
| SSP3102 | Lysosomal proteinase cathepsin B (CTSB) | 23,077 | 5.19 | 164 | 1314.6 1634.70 1823.90 |
ICEPGYSPTYK HYGYNSYSVSNSEK GQDHCGIESEVVAGIPR |
AAA52125 |
| SSP7711 | Keratin I (KRT1) | 66,038 | 8.15 | 68 | 1179.60 1475.70 |
YEELQITAGR FLEQQNQVLQTK |
NP_006112 |
Note: Protein identity was based on at >2 sequenced peptides from at least 2 separate MS/MS analysis. Internal standards: des-Arg1 Bradykinin, Angiotensin I and Glu1-Fibrino-peptide B (Monoisotopic MH+ m/z 904.4681, 1296.6853, 1570.6774, respectively) were used for mass calibration. The tandem mass spectra were collected in product ion mode on the peptide of interest. Measured peptide and fragment ion masses were used to search the NCBI and SWISS-PROT databases for protein identifications using the Mascot (www.matrixscience.com), with the following settings: mass tolerance at 50 ppm, one missed cleavage allowed, at least two matching peptides and searches limited to Homo sapiens species.