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. 2015 Oct 19;15:731. doi: 10.1186/s12885-015-1713-z

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© Zhao et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — CDKN1A was a putative target gene in regulating G1 phase arrest and DNA synthesis. a Cell cycle of A549 cells and A549/DDP cells was analyzed with FACS. The bar graphs showed the relative quantity of G1/S and G2/S ratio. b Cell cycle analysis of A549/DDP cells transfected with miRNA mimics, siRNAs and NC controls. c EdU assay of A549/DDP cells transfected with miRNA mimics, siRNAs and NC controls. Hoechst stained cells were blue and EdU add-in cells were rad. The bar graphs showed the relative quantity of cells incorporating EdU. At least 300 cells were counted per well. d Cell cycle analysis of A549 cells transfected with mRNA protectors and NC controls. e EdU assay of A549 cells transfected with mRNA protectors and NC controls. (n = 3, *P < 0.05)