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. 2015 Oct 20;5:35. doi: 10.1186/s13601-015-0079-2

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© Bégin et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — RT-PCR quantification of FOXP3 promoter and TSDR demethylation in CD4 T cell subpopulations. Culture of naïve CD4 T cells for 7 days with IL-2 and CD3/CD28 stimulation induced the demethylation of the FOXP3 promoter in the presence of TGF-β and/or retinoic acid (iTregs) but not with medium alone (a). FOXP3 promoter demethylation in absence of TSDR demethylation was also found to be a hallmark of CD45RA−CD25−CD4+ memory T cells and CD45RA−CD25^lowCD4+ “Treg-like” T conventional cells, but not of CD45RA+CD62L+CD4+ naïve or CD45RA+CD62L−CD4+ effector T cells (b)