Table 2.
Details of primary antibodies and detection systems
| Primary antibody | Source | Clone | Dilution | Company | Detection system |
|---|---|---|---|---|---|
| CD2AP | Ms IgG1 | B-4 | 1:1000 | SC | E |
| CD3 | Rb mAb | SP7 | 1:100 | TS | E |
| CD3 (IF) | Rb mAb | SP7 | 1:100 | TS | Rb FITC |
| CD4 | Ms IgG1 | 4B12 | 1:50 | NL | NP |
| CD8 | Ms IgG1 | C8/144B | 1:30 | DK | E |
| CD11c | Ms IgG2 | 5D11 | 1:50 | Mo | E |
| CD20 | Ms IgG2a | L26 | 1:250 | DK | E |
| CD45 | Ms IgG1 | X16/99 | 1:500 | NL | NP |
| CD56 | Ms IgG1 | 123C3.D5 | 1:30 | TS | E |
| CD56 (IF) | Ms IgG1 | 123C3.D5 | 1:30 | TS | Ms biot/Sav-TXR |
| CD66b | Ms IgM | G10F5 | 1:200 | Bi | NP |
| CD68 | Ms IgG3 | PG-M1 | 1:200 | DK | E |
| CD117 | Rb pAb | polyclonal | 1:50 | DK | E |
| CD123 | Ms IgG2a | 7G3 | 1:50 | BD | NP |
| CD138 | Ms IgG1 | MI15 | 1:100 | DK | E |
| CD163 | Ms IgG1 | 10D6 | 1:50 | TS | E |
| CD207 (Langerin) | Ms IgG2b | 12D6 | 1:150 | VL | E |
| CD303 (BDCA2) | Ms IgG1 | 124B3.13 | 1:75 | De | NP |
| Calretinin | Rb pAb | polyclonal | 1:200 | I | E |
| Neutrophil Elastase | Ms IgG1 | NP57 | 1:150 | DK | NP |
| Estrogen Receptor | Rb mAb | SP1 | 1:100 | TS | E |
| Foxp3 biot | Rat IgG2a | FJK-16s Set | 1:200 | eB | RP |
| Granzyme B | Ms IgG2a | 11F1 | 1:100 | NL | NP |
| Perforin | Ms IgG1 | 5B10 | 1:20 | NL | E |
| Progesterone Receptor | Ms IgG1 | PgR | 1:200 | DK | E |
| T-bet | Ms IgG1 | 4B10 | 1:50 | SC | NP |
| TCRβF1 | Ms IgG1 | 8A3 | 1:30 | TS | NP |
| TCRCγM1 | Ms IgG1 | γ3.20 | 1:400 | TS | NP |
IF: immunofluorescence; Rb mAb/pAb: rabbit monoclonal or polyclonal antibody; Ms: mouse antibody; biot: biotinylated antibody.
Detection system: E: EnVision (Dako); Rb FITC: Swine anti-rabbit FITC (Dako); NP: Novolink polymer (Novocastra Laboratories); Ms biot: goat anti-mouse biotynilated IgG1, Sav-TXR: Streptavidin TXR-conjugated (Southern Biotecnology*); RP: Rat-on-mouse HRP-polymer (Biocare Medical).
Company addresses: *SouthernBiotech, Birmingham, AL, USA; SC: Santa Cruz Biotechnology, Santa Cruz, CA, USA; TS: Thermo Scientific, Fremont, CA, USA; NL: Novocastra Laboratories, Newcastle upon Tyne, UK; DK: Dako, Glostrup, Denmark; Mo: Monosan, Uden, The Netherlands; Bi: BioLegend, San Diego, CA, USA; BD: BD Biosciences, San Jose, CA, USA; VL: Vector Laboratories, Burlingame, CA, USA; De: Dendritics, Lyon, France; I: Invitrogen, Carlsbad, CA, USA; eB: eBioscience, San Diego, CA, USA.