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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Dev Dyn. 2015 Jul 16;244(8):935–947. doi: 10.1002/dvdy.24285

Figure 3. TPEN exposure results in developmental arrest of cleavage stage preimplantation embryos.

Figure 3

1C (A), 2C (B), and 4C (C) embryos were cultured in the presence of 0 μM (white bar), 1 μM (black bar), or 10 μM TPEN (grey bar) for 24 h and developmental progression was scored by morphology. The percentage of embryos at each stage of development per treatment condition was calculated. Experiments were performed in triplicate with a minimum of 20 embryos per group. Results are reported as the mean ± SEM. Asterisks represent statistical significance as compared to control embryos at that particular developmental stage (**p<0.001). The schematics below each graph depict the experimental timing and expected developmental stage for control embryos. 1C = one-cell, 2C = two-cell, 4C = four-cell, 8C+ = eight-cell, morula and early blastocyst, dpc = days post coitus.