. Author manuscript; available in PMC: 2016 Aug 1.

Published in final edited form as: Dev Dyn. 2015 Jul 16;244(8):935–947. doi: 10.1002/dvdy.24285

Table 2.

Summary of numerical values for percentages of embryos at each stage of development in the presence of metal chelators.

		Total no. of embryos	Percent embryos arrested ± SEM¹	Percent embryos progressed ± SEM²
1C culture	Control	35	5.0 ± 5.0	95.0 ± 5.0
	TPEN	35	90.0 ± 10.0^**	10.0 ± 10.0^**
	Neocuproine	35	0.0 ± 0.0	100.0 ± 0.0
	TM	34	2.5 ± 2.5	97.5 ± 2.5
2C culture	Control	62	0.0 ± 0.0	100.0 ± 0.0
	TPEN	92	41.4 ± 10.2^**	58.6 ± 10.2^**
	Neocuproine	92	1.6 ± 1.6	98.4 ± 1.6
	TM	65	1.6 ± 1.6	98.4 ± 1.6
4C culture	Control	31	0.0 ± 0.0	100.0 ± 0.0
	TPEN	30	88.9 ± 11.1^**	11.1 ± 11.1^**
	Neocuproine	29	0.0 ± 0.0	0.0 ± 0.0
	TM	30	11.1 ± 11.1	88.9 ± 11.1

All embryos were cultured for 24 h. 1C = one-cell, 2C = two-cell, 4C = four-cell, TM = tetrathiomolybdate. Asterisks represent statistical significance as compared to control embryos

^**

p<0.001

Embryos were scored as arrested if, after 24h of culture, they were one-cell embryos for the 1C culture, two-cell embryos for the 2C culture, and four-cell embryos for the 4C culture.

Embryos were scored as progressed if, after 24h of culture, they were two-to-three-cell embryos for the 1C culture, four-to-eight-cell embryos for the 2C culture, and eight-cell or beyond embryos for the 4C culture.