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. Author manuscript; available in PMC: 2015 Nov 7.
Published in final edited form as: Oncogene. 2014 Jun 30;34(19):2538–2545. doi: 10.1038/onc.2014.171

Figure 1. Cables1 regulates protein levels of p21.

Figure 1

(A, B) Overexpressing Cables1 increases p21 at the protein level, but not at the mRNA level. HEK293T and H1299 cells were transfected with Venus and Venus-Cables1, then after 2 days, cells were lysed. Proteins were examined by Western blot, and mRNAs were examined by RT-PCR. (C, D) Knockdown of Cables1 decreases p21 at the protein level, but not at the mRNA level. HEK293T and H1299 cells were transfected with pLKO.1 Cables1 shRNA vectors and selected with puromycin (2 µg/ml) for 7 days, then cells were lysed. Proteins were examined by Western blot, and mRNAs were examined by RT-PCR.