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Multicompartmentalized catalytic synthetic cells. Initially, encapsulation of enzymes occurs in polystyrene-b-poly(3-(isocyano-lalanyl-amino-ethyl)-thiophene) (PS-b-PIAT) nanoreactors, followed by mixing of the artificial organelles, cytosolic enzymes, and reagents, and subsequent encapsulation of the mixture in polybutadiene-b-poly(ethylene oxide) (PB-b-PEO) vesicles to create the functional synthetic cell, inside which enzymatic multicompartment catalysis occurs.
