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. Author manuscript; available in PMC: 2015 Oct 24.
Published in final edited form as: Cell Rep. 2015 Sep 24;13(2):337–349. doi: 10.1016/j.celrep.2015.08.075

Figure 1. LR asymmetric gene expression at the Pitx2 locus.

Figure 1

(A) Pitx2 initiates gut looping directed by LR changes in the DM cellular architecture (tan, left; green, right). DM microarray (LE, left epithelium; LM, left mesenchyme; RM, right mesenchyme; RE, right epithelium) identifies genes linked to Pitx2 with right side-restricted expression, validated in (B) via whole mount ISH (double labeling Pitx2, magenta; right-specific genes, blue) at HH21 (DM), at HH17 (lateral mesoderm, DM precursor, arrows) and at HH12 (sinus venosus of the primitive heart, arrows). (C) Pitx2 locus conservation in chicken, mouse, and human (dashed line, human ARS deletion). Note, human locus is inverted relative to its orientation on chr4. (D) e926 directs left-specific reporter gene expression (LacZ) in the left DM of transgenic mouse embryos. Scale bars: (B) 100μm, HH21 and HH17, 500μm, HH12; (D) 500μm, whole mount, 25μm, section. See also Figure S1.