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. 2015 Oct 20;10(10):e0140784. doi: 10.1371/journal.pone.0140784

Fig 1. Deletion of S. pyogenes sortase results in aberrant cellular morphology.

Fig 1

Wild type S. pyogenes D471, sortase mutant AR01, complemented AR01+pAR107, and a variant of AR01 that was passaged 6 times in TH+Y, were diluted from overnight cultures 1:100 into fresh media (containing spectinomycin for AR01+pAR107), grown to log phase, and fixed. The cells were processed for transmission and scanning electron microscopy as described in the Material and Methods section. Scale bars represent 500 nm.