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. 2015 Oct 15;29(20):2108–2122. doi: 10.1101/gad.268979.115

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© 2015 Costello et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 3. — Lhx1 function is essential for midline morphogenesis. (A) Shh expression is absent at E7.5 in the node progenitors and subsequently, at E7.75, becomes discontinuous and fails to extend anteriorly. By E8.5, the anterior midline is severely disturbed. (B) At EHF stages, Brachyury is normally expressed in the PS, node (asterisk), and anterior midline, whereas, in Lhx1ΔEpi embryos, midline expression is patchy. The observed speckles are background staining. (C) Frontal view at slightly later stages (four- to five-somite stage) reveals discontinuous Brachyury staining in Lhx1ΔEpi mutants. (D) Sox17 (red) and Foxa2 (green) double staining of the emerging midline. In Lhx1ΔEpi embryos, midline Foxa2 staining is reduced, and the Sox17⁺ endoderm obscures the midline. (E) Analysis of Afp:GFP reporter expression in Lhx1ΔEpi embryos reveals defective node and midline development. The endoderm-obstructing node and midline emergence is GFP⁺ VE.