Figure 5.
Constitutive BAK oligomerization correlates with relative BAK content. (A) Whole-cell lysates, along with different concentrations of Molt3, were blotted with the indicated antibodies. Orange and green letters indicate the cell lines in the orange and green boxes, respectively, in Figure 2. (B–D) The percentage of BAK that is constitutively oligomerized (% BAK oligomer), calculated as shown in Supplemental Figure S2, versus the amount of BIMEL (B), BID (C), or BAK (D) in each line relative to an equal amount of Molt3 whole-cell lysate, determined as described in the Materials and Methods.