Fig. 2.

miR-320b overexpression inhibits CRC cells growth in vitro. a miR-320b expression was quantified by qRT-PCR analysis 48 h. after transfection of miR-320b mimics (miR-320b) and negative control oligonucleotides (NC) in HCT-116 and SW-480 cells. b HCT-116 or SW-480 cells were transfected with miR-320b or NC. Cell proliferation was measured using the MTT assay after culturing for 72 h. c Cell-cycle distribution was analyzed by FACS analysis. HCT-116 and SW-480 cells were transfected with miR-320b or NC. Nocodazole (25 ng/ml) was added 24 h after transfection for another 16 h, then the supernatant was replaced by fresh medium for 6 h. Data analyzed using Student’s t-test.*p < 0.05, **p < 0.01