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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Circ Cardiovasc Genet. 2015 Sep 11;8(5):717–726. doi: 10.1161/CIRCGENETICS.115.001079

Table 3.

Allele-specific NLRC4 isoform expression in relation to the rs385076 genotype.

Association between mRNA (deltaCt) and rs385076 T alleles Association between mRNA (deltaCt) and log(IL-18)
Isoforms Description delta Ct Beta SE P N Beta SE P N
2, 4 Including exon 2 5.1±1.4 0.305 0.047 2.0×10−10 1,175 0.009 0.008 0.254 1,459
1, 3 Excluding exon 2 5.2±0.8 −0.103 0.030 7.4×10−4 1,171 0.027 0.014 0.049 1,438
1, 2, 3, 4 Total NLRC4 mRNA 3.2±1.3 0.134 0.050 6.9×10−3 1,180 0.014 0.008 0.092 1,467

Expression levels of mRNA are represented as deltaCt values (deltaCt(transcript) = Ct(transcript) – Ct(GAPDH)), which are normalized for the housekeeping gene GAPDH. Beta estimates were calculated in a linear mixed model and refer to the change in deltaCt for each T allele of rs385076 and the change in log(IL-18), respectively. Therefore, a negative beta implies an increase in gene expression. N refers to the number of individuals with non-missing values used for a test.