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. Author manuscript; available in PMC: 2015 Oct 24.
Published in final edited form as: Cell Rep. 2015 Oct 8;13(3):451–459. doi: 10.1016/j.celrep.2015.09.017

Figure 2. Identification of the PARP1 Binding Site in TIMELESS.

Figure 2

(A) HEK293T cells were transfected with either an empty vector (EV), FLAG-tagged TIMELESS, or FLAG-tagged TIMELESS mutants as indicated. Asterisks indicate the insertion of a STOP codon after the numerically specified codon. 24 hr post-transfection, WCEs were subjected to IP with α-FLAG resin and immunoblotted as indicated.

(B) Schematic representation of TIMELESS mutants tested for binding to PARP1. TIMELESS mutants that interacted with endogenous PARP1 are designated with the symbol (+); those unable to co-precipitate PARP1 are designated with the symbol (−).

(C) The experiment was performed as in (A), except that different TIMELESS mutants were used.