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. 2015 Oct 20;10(10):e0140743. doi: 10.1371/journal.pone.0140743

Fig 5. Deletion of clpV increases intracellular survival in vitro.

Fig 5

(A) RAW264.7 macrophages were infected with RB50 (blue, n = 4) or RB50ΔclpV (red, n = 4) at an MOI of 100 and bacterial invasion and intracellular survival was determined at 1, 24, and 48 hour after addition of gentamicin. The experiment was conducted five times with similar results and a representative dataset is shown. (B) The cytotoxicity of RAW264.7 macrophages infected with RB50 (blue) or RB50ΔclpV (red) at an MOI of 100 was determined 1 hour and 24 hours after gentamicin application. The average percent cytotoxicity of four wells in three different experiments was measured by (LDH release from a well / LDH release from positive control well) x 100 ±SE is shown. * denotes p value <0.05. Grey line indicates limit of detection.