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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Am J Hematol. 2015 Oct 8;90(11):1027–1035. doi: 10.1002/ajh.24133

Figure 3. Vascular changes following induced hemarthrosis in FVIII-deficient mice.

Figure 3

The right knees of FVIII-deficient mice were subjected to subpatellar puncture. Vascularity changes were assessed histologically following Safranin-0-Green staining at 40-fold magnification. Findings were recorded at 2, 4 and 10 weeks post-injury and compared to baseline as well as to the uninjured left knee (n=5–6 per group). Error bars represent standard of the mean. A) Total vessel count per joint in synovial and stromal tissue between fernoral and tibial growth plates. B) Individual diameters of all vessels in each joint. C) Percentage of vessels in each joint with a diameter ≥ 20 µm. D) Representative examples stained with Safranin-O, depicting the increase of vessel diameter in the right and also left knee 2 weeks after injury compared to baseline in 6 different mice. Statistical significance was determined by Kruskal Wallis test with Dunns multiple comparison test. * indicates statistical significance (p ≤ 0.05). Error bars represent the SEM.