Figure 2. Axin2-expressing cells in the prepubescent prostate gland contribute to luminal lineage expansion.

(A): A scheme of experimental designs to label and analyze prepubescent Axin2-expressing cells in the prostate of Axin2^CreERT2/+;Rosa^mTmG/+ mice. Expression of mT (red) and mGFP (green) protein in different prostatic lopes of Axin2^CreERT2/+;Rosa^mTmG/+ mice at P19 (B–D), P60 (G–I), and P120 (L–N). Co-immunofluorescence staining of mGFP (green) and K5 (red) or K8, as well as DAPI was preformed in the prostate of Axin2^CreERT2/+;Rosa^mTmG/+ mice. Representative images in DLP at P19 (E–F), P60 (J–K), and P120 (O–P) were shown. Percentages of mGFP+ cells in K8+ (Q) and in K5+ cells (R) at P19 (white bars), P60 (red bars), or P120, after a round of regression/regeneration (blue bars) in different prostatic lopes. Error bars indicate standard deviation. *, P < 0.01 by ANOVA. Scale bars, 20 µm.