ALK inhibition attenuates ethanol-stimulated increase in pERK in the amygdala. (a–d) Images showing pERK staining in the mouse CeA after saline (a, c) or 3 g/kg ethanol (b, d) treatment and vehicle (a, b) or TAE684 treatment (c, d). (e) Quantification of pERK intensity using Image J. There were significant effects of ethanol and TAE684 treatments. (f) pERK intensity after injection of 2 g/kg ethanol or saline. (g) pERK staining in mice treated with 2 g/kg ethanol and TAE684 or vehicle. Data are presented as mean ± S.E.M. ***p <0.001, ****p < 0.0001. Abbreviations: Sal, saline; Veh, vehicle; EtOH, ethanol; CeA, central nucleus of the amygdala; BLA, basolateral amygdala; ec, external capsule; ic, internal capsule. Scale bar, 200 μm.