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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Circ Cardiovasc Genet. 2015 Jul 31;8(5):665–676. doi: 10.1161/CIRCGENETICS.115.001138

Figure 4.

Figure 4

SOD1 overexpression rescues diabetes-increased canonical Wnt antagonists and restores the expression of key canonical Wnt intermediates. A, mRNA abundance of the three canonical Wnt antagonists. B, representative western blots of phosphorylated and non-phosphorylated Dvl2, p-GSK3β and β-catenin. The dot graphs showed the quantification of the immunoblotting. In A and B, three hearts from embryos of three dams (n = 3) per group were analyzed. C, images of β-catenin immunostaining. Rabbit normal IgG served as controls. Red signal was β-catenin and cell nuclei were stained by DAPI (Blue). Three E12.5 hearts from embryos of three dams (n = 3) per group, and three serial sections per heart were analyzed, and similar results were obtained. Bars = 150 µm. * in A, B indicate significant difference compared to other groups. ND: nondiabetic; DM: diabetic mellitus; WT: wild-type hearts; SOD1: SOD1 overexpressing hearts.