Fig 3. Ov-GRN-1 stimulated wound repair in vivo.
(A) Sequential images over four days of healing wounds revealed the response of mice to treatment with recombinant rOv-GRN-1 or rTRX control; skin-deep wounds made with a 5 mm diameter biopsy punch between the ears of Balb/c mice. Minor modifications (brightness, contrast, cropping) were made to aid viewing. (B) The rate of wound healing over four days was measured; wound closure was determined electronically from photographs by measuring wound areas with ImageJ software. To aid viewing, curves have been shifted left or right marginally to minimize error bar overlap. (C) Assessment of the angiogenic properties of recombinant Ov-GRN-1 in the chorioallantoic membrane (CAM) assay. The numbers of blood vessels in quail eggs that grew on 0.5 cm2 filter paper soaked in rOv-GRN-1 or vehicle (control) were ascertained after incubation for 15 hours. Data points are the averages of two experiments with 3–5 biological replicates displayed with SEM bars. * = P<0.05, ** = P<0.01, *** = P<0.001, **** = P<0.0001, ns = not significant.