Skip to main content
. 2015 Oct 20;9(10):e0004168. doi: 10.1371/journal.pntd.0004168

Fig 2. Purity and antigenicity of purified recombinant proteins.

Fig 2

SDS-PAGE of the three recombinant proteins. A. Tc-CTL-1; B. Tc-TES-26; C. Tc-MUC-3. The recombinant protein samples at a concentration of: 1–6.25 ng/mm; 2–3.125 ng/mm; 3–1.6 ng/mm, and 4–0.8 ng/mm was treated with SDS and heated at 65°C for 15 minutes, separated and analyzed using SDS gel electrophoresis and western blotting. Two gels were transferred to nitrocellulose membranes and probed with a strong EIA positive Toxocara human sera pool, a negative human serum sample diluted 1:100 in PBS/Tween 0.3%/5% milk, and one gel was incubated with protein staining, Colloidal Gold Total Protein Stain (Bio-Rad, Cat. # 170–6527). M = Precision Plus Protein Dual Xtra Standards (Biorad, Cat. #161–0377)