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. 2015 Oct 21;8:546. doi: 10.1186/s13071-015-1170-1

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© Krajacich et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Immunoblot analysis of serum from mice immunized with only the adjuvant (a) or purified recombinant Vtp 6 with adjuvant (b). Antigen was purified Vtp 6 and serum samples were tested at 1:200 dilutions. Five mice were examined in each group (M1 – M5). Molecular mass standards on left in kDa. Arrow indicates the primary Vtp protein, slightly larger than the native protein because of the 6-histidine tag; larger aggregates of the protein are above