Fig. 6.

Silencing of SlHUB1 and SlHUB2 resulted in reduced cell wall thickness but increased callose accumulation after B. cinerea infection. Two-week-old seedlings were infiltrated with agrobacteria carrying pTRV2-SlHUB1, pTRV2-SlHUB2, pTRV2-SlMED21 or pTRV2-GUS constructs. a, b Representative TEM photos showing the cell wall (a) and the thickness of cell wall (b) in pTRV2-SlHUB1-, pTRV2-SlHUB2- or pTRV2-GUS-infiltrated plants. Leaf samples were collected for TEM assays at 4 weeks after VIGS infiltration. Bars = 200 nm. The data represent mean ± SE from 20 samples. c Callose accumulation. The VIGS construct-infiltrated plants were inoculated with B. cinerea and at least 6 leaves from 6 individual plants were collected at 0 h and 24 h after inoculation for detection of callose accumulation. Upper row represents callose staining in mock-inoculated leaves whereas lower row represents callose staining in B. cinerea-inoculated leaves. Bars = 100 μm. The callose data shown in (d) were quantified using an image analysis program as described in Methods