Fig. 7.

Silencing of SlHUB1 and SlHUB2 attenuated the expression of phenylpropanoid pathway-related genes after B. cinerea infection. Two-week-old seedlings were infiltrated with agrobacteria carrying pTRV2-SlHUB1, pTRV2-SlHUB2, pTRV2-SlMED21 or pTRV2-GUS constructs and were inoculated with spore suspension of B. cinerea at 4 weeks after VIGS infiltration. At least 6 leaves from 6 individual plants were collected at 0 and 24 h after inoculation and used for analysis of gene expression. Data presented are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p < 0.05 level