Fig. 9.

Silencing of SlHUB1 and SlHUB2 affected the expression of SA-, JA- and ET-mediated signaling and responsive genes after Botrytis infection. Two-week-old seedlings were infiltrated with agrobacteria carrying pTRV2-SlHUB1, pTRV2-SlHUB2, pTRV2-SlMED21 or pTRV2-GUS construct and were inoculated with spore suspension of B. cinerea at 4 weeks after VIGS infiltration. At least 6 leaves from 6 individual plants were collected at 0 and 24 h after inoculation and used for analysis of gene expression. a Expression of SA-mediated signaling and responsive genes, (b) Expression of JA-mediated signaling and responsive genes and (c) Expression of ET-mediated signaling and responsive genes. Relative expression levels were shown as folds of the actin transcript values. Data presented in (b) are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p < 0.05 level