Skip to main content
. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Neurosci Res. 2015 Sep 14;93(12):1881–1890. doi: 10.1002/jnr.23669

Figure 2. SVCT2 is localized in presynaptic synaptosomes.

Figure 2

[A] Western immunoblots of cortical synaptosome preparations and fractionated samples. Samples containing 5 μg of protein were subjected to SDS-PAGE and immunoblotting. Samples were probed for the cytosolic protein, glyceraldehyde 3-phosphate dehydrogenase; for the presynaptic protein, vesicular monoamine transporter-2; and for two postsynaptic proteins, N-methyl-D-aspartate-1 and postsynaptic density protein-95. G-protein β subunit served as a loading control.[B] Samples containing 30 μg of protein were subjected to SDS-PAGE and immunoblotting under same fractionation conditions and probed for SVCT2. Bands in each lane [A and B] were normalized by GβP expression in the same blot and compared to the initial crude synaptosome preparation (first lane). Quantification of [C] GAPDH, [D] VMAT2, [E] NMDAR1, [F] PSD-95 and [G] SVCT2 are represented as the mean ± SEM, N=2. *P<0.05 where fractions are compared to control group (WHOLE) using two-tailed, unpaired T-test.

GAPDH, glyceraldehyde 3-phosphate dehydrogenase; VMAT2, vesicular monoamine transporter-2; N-methyl-D-aspartate-1, NMDAR1, postsynaptic density protein-95, PSD-95; G-protein β subunit, GβP