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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Neurosci Res. 2015 Sep 14;93(12):1881–1890. doi: 10.1002/jnr.23669

Figure 4. Time-dependent spontaneous VC Efflux.

Figure 4

Time course of spontaneous VC efflux. [A] Relative VC content over 30 min at 4°C (closed circles) and 37°C (open circles). The initial synaptosomal VC concentration was ∼250-300 nmol/g (n=6). [B] Logarithmic transformation of data in panel A. [C] Relative VC content over 30 min of efflux at 37°C with (open circles) and without (closed circles) 1 mM sulfinpyrazone (n=4). [D] Logarithmic transformation of data in panel C. [E] Relative VC content over 30 mins of efflux at 37°C with (open circles) and without (closed circles) 100 μM phloretin (n=4). [F] Logarithmic transformation of data in panel C. In all the log-transformed panels, an asterisk (*) denotes a significant difference between the slopes of the two lines (p<0.05).

SPZ, sulfinpyrazone; PHL, phloretin; VC, Vitamin C