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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Neurosci Res. 2015 Sep 14;93(12):1881–1890. doi: 10.1002/jnr.23669

Table 1. Antibodies Used in Methods.

Antibody Name Immunogen Structure Manufacturer, Catalog #, RRID, Species, Type (mono- poly- clonal) Concentration Used
Primary
SVCT2 Epitope mapping near the N-terminus of SVCT2 of rat origin Santa Cruz Biotechnology, SC-9926, RRID: AB_661205, goat polyclonal 1:200
VMAT2 Epitope corresponding to amino acids 44-133 mapping near the N-terminus of synaptic vesicle monoamine transporter 2 (VMAT 2) of human origin Santa Cruz Biotechnology, SC-15314, RRID: AB_2187867, rabbit polyclonal 1:200-400
NET Raised against a peptide (amino acids 05-17) coupled to KLH by the addition of an C-terminal cysteine MAb Technologies, NET05, mouse monoclonal
Actin Epitope mapping at the C-terminus of Actin of human origin Santa Cruz Biotechnology, SC-1616, RRID: AB_10160631, goat polyclonal 1:400
GβP Epitope mapping at the C-terminus of Gβ of mouse origin Santa Cruz Biotechnology, SC- 378, RRID: AB_631542, rabbit polyclonal 1:10000
GAPDH Antibody clone number 6C5, Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle. Milllipore, MAB-374, mouse monoclonal 1:20000
NMDAR1 Rat NMDAR1 aa. 660-811 Recombinant Protein BD Biosciences, 556308, RRID: AB_396353, Mouse monoclonal 1:2000
PSD-95 Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (also known as Postsynaptic density protein 95, Disks large homolog 4, Synapse-associated protein 90, DLG4, Dlgh4 and SAP-90, accession number P78352). Clone K28/43 Neuromab, 75-028, RRID: AB_2307331, mouse monoclonal 1:20000
Secondary
Anti-rabbit IgG-conjugated horseradish peroxidase Cruz Marker™ compatible secondary antibodies recognize an epitope common to each of the Cruz Marker™ molecular weight standards Santa Cruz Biotechnology, SC-2030, RRID: AB_631747, Goat polyclonal 1:20000-30000
Anti-goat IgG-conjugated horseradish peroxidase Cruz Marker™ compatible secondary antibodies recognize an epitope common to each of the Cruz Marker™ molecular weight standards Santa Cruz Biotechnology, SC-2020, RRID: AB_631728, donkey polyclonal 1:5000-10000
Anti-Rabbit IgG-conjugated horseradish peroxidase Rabbit IgG PerkinElmer, NEF812001EA, goat polyclonal 1:20000
Anti-mouse IgG- Mouse-IgG PerkinElmer, NEF822E001EA, goat 1:10000-20000
Conjugated horseradish peroxidase polyclonal